rabbit anti-cgrp polyclonal antibody  (Peninsula Laboratories)

Journal: Redox biology

Article Title: Lipoxin A 4 ameliorates knee osteoarthritis progression in rats by antagonizing ferroptosis through activation of the ESR2/LPAR3/Nrf2 axis in synovial fibroblast-like synoviocytes.

doi: 10.1016/j.redox.2024.103143

Figure Lengend Snippet: Fig. 2. Downregulation of ESR2, LPAR3, and GPX4 and upregulation of HO-1 and CGRP levels in synovial tissue with increasing Kellgren–Lawrence (KL) grades. (A) Immunohistochemical staining results for ESR2, LPAR3, GPX4, HO-1, and CGRP. (B–F) Statistical analysis of the immunohistochemical staining results. A comparison of all KL grades with grade 0 is shown (*P < 0.001, *aP = 0.004, *bP = 0.002, *cP = 0.039, *dP = 0.03, *eP = 0.009, ANOVA). Data are expressed as the mean ± 95% confidence interval; n = 3 patients per group.

Article Snippet: Then, the following primary antibodies were added: rabbit polyclonal anti-ESR2 antibody (14007-1-AP, 1:1000; Proteintech), rabbit polyclonal anti-LPAR3 antibody (orb394997, 1:1000; Biorbyt), rabbit monoclonal anti–HO–1 antibody (ab189491, 1:2000; Abcam), rabbit monoclonal anti-GPX4 antibody (ab125066, 1:500; Abcam), rabbit monoclonal anti-SOD1 antibody (ab308181, 1:100; Abcam), rabbit polyclonal anti-CGRP antibody (16630-1-AP, 1:100; Proteintech), overnight at 4 ◦C in a cold room.

Techniques: Immunohistochemical staining, Staining, Comparison

Journal: Heliyon

Article Title: Urothelial bladder afferents selectively project to L6/S1 levels and are more peptidergic than those projecting to the T13/L1 levels in female rats

doi: 10.1016/j.heliyon.2023.e18495

Figure Lengend Snippet: Cell Count; Cell Diameters (μm±SEM) of bPANs.

Article Snippet: Tissues were then incubated overnight at 4°C in blocking buffer containing the following primary antibodies: polyclonal rabbit anti-CGRP primary antibody (1:2000; ImmunoStar cat. #24112) or polyclonal guinea pig anti-SP primary antibody (1:1000; Abcam cat. #10353).

Techniques: Cell Counting, Labeling, Staining

Journal: Heliyon

Article Title: Urothelial bladder afferents selectively project to L6/S1 levels and are more peptidergic than those projecting to the T13/L1 levels in female rats

doi: 10.1016/j.heliyon.2023.e18495

Figure Lengend Snippet: Representative examples of dorsal root ganglion neurons retrogradely labeled with Fast Blue and DiI, and immunohistochemically stained with antibodies against CGRP (Panel A) and SubP (Panel B). White arrows indicate DiI-labeled neurons. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Tissues were then incubated overnight at 4°C in blocking buffer containing the following primary antibodies: polyclonal rabbit anti-CGRP primary antibody (1:2000; ImmunoStar cat. #24112) or polyclonal guinea pig anti-SP primary antibody (1:1000; Abcam cat. #10353).

Techniques: Labeling, Staining